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human liver carcinoma cell line hepg2 (ATCC)

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ATCC human liver carcinoma cell line hepg2

Optimization of cell-related conditions for the RSV mRNA potency assay. ( A ) Dose–response curves of RSV mRNA transfection in different cell lines (293T, Vero, HEp-2, <t>HepG2,</t> and A549). Cells were transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well), and RSV prefusion F protein expression was measured by ELISA as OD 450 –OD 630 . The corresponding EC 50 and slope values are summarized in the inset table. ( B ) Effect of HepG2 cell seeding density on RSV prefusion F protein expression. HepG2 cells were seeded at 0.5 × 10 5 , 1.0 × 10 5 , or 2.0 × 10 5 cells/well and transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well). Protein expression was measured by ELISA as OD 450 –OD 630 .

Human Liver Carcinoma Cell Line Hepg2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 29907 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human liver carcinoma cell line hepg2 - by Bioz Stars, 2026-09

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1) Product Images from "Establishment and Validation of a Cell-Based Relative Potency Method for Respiratory Syncytial Virus mRNA Vaccine Drug Substance"

Article Title: Establishment and Validation of a Cell-Based Relative Potency Method for Respiratory Syncytial Virus mRNA Vaccine Drug Substance

Journal: Vaccines

doi: 10.3390/vaccines14050401

Figure Legend Snippet: Optimization of cell-related conditions for the RSV mRNA potency assay. ( A ) Dose–response curves of RSV mRNA transfection in different cell lines (293T, Vero, HEp-2, HepG2, and A549). Cells were transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well), and RSV prefusion F protein expression was measured by ELISA as OD 450 –OD 630 . The corresponding EC 50 and slope values are summarized in the inset table. ( B ) Effect of HepG2 cell seeding density on RSV prefusion F protein expression. HepG2 cells were seeded at 0.5 × 10 5 , 1.0 × 10 5 , or 2.0 × 10 5 cells/well and transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well). Protein expression was measured by ELISA as OD 450 –OD 630 .

Techniques Used: Potency Assay, Transfection, Expressing, Enzyme-linked Immunosorbent Assay

Cell Culture:

Article Title: Blocked metabotropic glutamate receptor 5 enhances chemosensitivity in hepatocellular carcinoma and attenuates chemotoxicity in the normal liver by regulating DNA damage.
Article Snippet: DNA damaging agents are used as chemotherapeutics in many cancers, including hepatocellular carcinoma (HCC).. However, they are associated with problems such as low sensitivity to chemotherapy and the induction of liver injury, underscoring the need to identify new therapies.. Here, we investigated the differential regulatory effect of metabotropic glutamate receptor 5 (mGlu5) on chemosensitivity in HCC and chemotoxicity to the normal liver.

Article Title: Blocked Metabotropic Glutamate Receptor 5 Enhances Chemosensitivity in Hepatocellular Carcinoma and Attenuates Chemotoxicity in the Normal Liver by Regulating DNA Damage
Article Snippet: Abbreviations: HCC, hepatocellular carcinoma; mGlu5, metabotropic glutamate receptor 5; PD, Parkinson's disease; MPEP, 2-methyl-6-(phenylethynyl) pyridine; MMS, methyl methanesulfonate; BAPTA, 1,2-bis-(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; CNS, central nervous system; MAPK, mitogen-activated protein kinase; CHPG, (RS)-2-Chloro-5hydroxyphenylglycine *Correspondence: Hong Zhang, Department of Neurobiology, School of Basic Medical Sciences, Beijing Key Laboratory of Neural Regeneration and Repair, Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, China.. Telephone: +86 010 8395 0379.. E-mail address: hzhang@ccmu.edu.cn (H Zhang) 1 Blocked metabotropic glutamate receptor 5 enhances chemosensitivity in hepatocellular 2 carcinoma and attenuates chemotoxicity in the normal liver by regulating DNA damage 3 Hui-Min Yang a, Tian-Zhong Hou a, Ya-Nan Zhang a, Shu-Dong Zhao a, b, Yong-Le Wu c, 4 Hong Zhang a,* 5 a Department of Neurobiology, School of Basic Medical Sciences, Beijing Key Laboratory of 6 Neural Regeneration and Repair, Beijing Institute for Brain Disorders, Capital Medical 7 University, Beijing 100069, China 8 b Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing 9 Advanced Innovation Center for Biomedical Engineering, School of Engineering Medicine, 10 Beihang University, Beijing, 100083, China 11 c Center of Hepatic and Digestive Disease, Beijing YouAn Hospital, Capital Medical 12 University, Beijing 100069, China This preprint research paper has not been peer reviewed.

Article Title: Integrated safety and microbiota profiling of fulvic acid formulations across in vitro and in vivo models.
Article Snippet: .. The L929 mouse skin fibroblasts (CCL-1, ATCC, USA) and THP1-BlueTM NF-κB reporter cells (InvivoGen, USA) were cultured in RPMI-1640 medium (Gibco, USA), human colorectal adenocarcinoma LoVo cells (CCL-229, ATCC, USA) were cultured in DMEM/F12 medium (Gibco, USA), and human liver carcinoma HepG2 cells (HB-8065, ATCC, USA) were maintained in DMEM (Gibco, USA). .. All culture media were supplemented with 10% heat-inactivated fetal bovine serum (FBS; HyClone, USA), penicillin (100 U/mL), and streptomycin (100 μg/mL) (Thermo Fisher Scientific, USA), and cells were maintained at 37°C in a humidified atmosphere containing 5% CO2.

Article Title: Integrated safety and microbiota profiling of fulvic acid formulations across in vitro and in vivo models
Article Snippet: .. The L929 mouse skin fibroblasts (CCL-1, ATCC, USA) and THP1-BlueTM NF-κB reporter cells (InvivoGen, USA) were cultured in RPMI-1640 medium (Gibco, USA), human colorectal adenocarcinoma LoVo cells (CCL-229, ATCC, USA) were cultured in DMEM/F12 medium (Gibco, USA), and human liver carcinoma HepG2 cells (HB-8065, ATCC, USA) were maintained in DMEM (Gibco, USA). .. All culture media were supplemented with 10% heat-inactivated fetal bovine serum (FBS; HyClone, USA), penicillin (100 U/mL), and streptomycin (100 μg/mL) (Thermo Fisher Scientific, USA), and cells were maintained at 37 °C in a humidified atmosphere containing 5% CO2.

Article Title: Selective Photoaffinity Probe for Monitoring Farnesoid X Receptor Expression in Cultured Cells.
Article Snippet: Farnesoid X receptor (FXR), a member of the nuclear receptor superfamily, is a vital ligand-activated transcriptional factor, which is highly expressed in the liver, intestine, and adrenal gland.. However, FXR homeostasis is influenced by many factors, such as diet and circadian rhythm, and the expression of FXR differs in diverse organs.. Currently, there is no method to monitor the FXR homeostasis in real time, which restricts us from further investigating the function of FXR under physiological and pathological conditions.

Article Title: Synthesis of Radioluminescent CaF 2 :Ln Core, Mesoporous Silica Shell Nanoparticles for Use in X-ray Based Theranostics
Article Snippet: .. Murine fibroblast (NIH 3T3) and human liver carcinoma (HepG2) cells (both sourced from ATCC, Manassas, VA, USA) were cultured in appropriate growth media and seeded at a density of approximately 10,000 cells per well into a 96-well plate. ..

Modification:

Negative Control:

Article Title: T-2 toxin and deoxynivalenol (DON) exert distinct effects on stress granule formation depending on altered activity of SIRT1.
Article Snippet: .. Human liver carcinoma HepG2 cells (ATCC, HB-8065) were treated with 0.01 % DMSO (negative control), 7 μM DON or 10 μM TSA and harvested after 24 h. Three biological replicates per treatment group were produced in this way. ..

Produced:

Derivative Assay:

Article Title: Brivanib Exhibits Potential for Pharmacokinetic Drug-Drug Interactions and the Modulation of Multidrug Resistance through the Inhibition of Human ABCG2 Drug Efflux Transporter and CYP450 Biotransformation Enzymes.
Article Snippet: Brivanib, a promising tyrosine kinase inhibitor, is currently undergoing advanced stages of clinical evaluation for solid tumor therapy.. In this work, we investigated possible interactions of this novel drug candidate with ABC drug efflux transporters and cytochrome P450 (CYP450) drug-metabolizing enzymes that participate in cancer multidrug resistance (MDR) and pharmacokinetic drug−drug interactions (DDIs).. First, in accumulation experiments with various model substrates, we identified brivanib as an inhibitor of the ABCB1, ABCG2, and ABCC1 transporters.

Stable Transfection:

Transduction:

Image Search Results

Journal: Vaccines

Article Title: Establishment and Validation of a Cell-Based Relative Potency Method for Respiratory Syncytial Virus mRNA Vaccine Drug Substance

doi: 10.3390/vaccines14050401

Figure Lengend Snippet: Optimization of cell-related conditions for the RSV mRNA potency assay. ( A ) Dose–response curves of RSV mRNA transfection in different cell lines (293T, Vero, HEp-2, HepG2, and A549). Cells were transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well), and RSV prefusion F protein expression was measured by ELISA as OD 450 –OD 630 . The corresponding EC 50 and slope values are summarized in the inset table. ( B ) Effect of HepG2 cell seeding density on RSV prefusion F protein expression. HepG2 cells were seeded at 0.5 × 10 5 , 1.0 × 10 5 , or 2.0 × 10 5 cells/well and transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well). Protein expression was measured by ELISA as OD 450 –OD 630 .

Article Snippet: Cell lines used for mRNA transfection included the human liver carcinoma cell line HepG2 (American Type Culture Collection, ATCC, Manassas, VA, USA), human non-small cell lung carcinoma cell line A549 (ATCC), African green monkey kidney epithelial cell line Vero (ATCC), human epidermoid carcinoma type 2 cell line Hep-2 (ATCC), and human embryonic kidney cell line 293T (China Center for Type Culture Collection, CCTCC, Wuhan, China).

Techniques: Potency Assay, Transfection, Expressing, Enzyme-linked Immunosorbent Assay

Genotoxic effect observed on LoVo (left) and HepG2 (right) cell lines after 24 h incubation with tested preparations containing fulvic acid (MLG-50, MLG-A50) in the range of 1:8192–1:32768. Nucleus damage presented as tail DNA [%] (Lucia Comet Assay™, Laboratory Imaging). PBS was used as a non-treated control (NTC), and 50 µg/mL H 2 O 2 as a treated control. For each independent experiment, 50–100 nucleoids were randomly analyzed per condition. Data are shown as mean ± standard error of the mean (SEM) from four independent experiments ( n = 4). Statistics were performed using one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test; * indicates statistical significance versus the non-treated control (NTC) ( p < 0.05). Criteria for interpretation of damage levels were as follows: ≤5%—no or minor damage; 5–20%—low damage; 20–40%—moderate damage; 40–75%—high damage; >75%—severe damage.

Journal: Scientific Reports

Article Title: Integrated safety and microbiota profiling of fulvic acid formulations across in vitro and in vivo models

doi: 10.1038/s41598-026-37331-2

Figure Lengend Snippet: Genotoxic effect observed on LoVo (left) and HepG2 (right) cell lines after 24 h incubation with tested preparations containing fulvic acid (MLG-50, MLG-A50) in the range of 1:8192–1:32768. Nucleus damage presented as tail DNA [%] (Lucia Comet Assay™, Laboratory Imaging). PBS was used as a non-treated control (NTC), and 50 µg/mL H 2 O 2 as a treated control. For each independent experiment, 50–100 nucleoids were randomly analyzed per condition. Data are shown as mean ± standard error of the mean (SEM) from four independent experiments ( n = 4). Statistics were performed using one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test; * indicates statistical significance versus the non-treated control (NTC) ( p < 0.05). Criteria for interpretation of damage levels were as follows: ≤5%—no or minor damage; 5–20%—low damage; 20–40%—moderate damage; 40–75%—high damage; >75%—severe damage.

Article Snippet: The L929 mouse skin fibroblasts (CCL-1, ATCC, USA) and THP1-BlueTM NF-κB reporter cells (InvivoGen, USA) were cultured in RPMI-1640 medium (Gibco, USA), human colorectal adenocarcinoma LoVo cells (CCL-229, ATCC, USA) were cultured in DMEM/F12 medium (Gibco, USA), and human liver carcinoma HepG2 cells (HB-8065, ATCC, USA) were maintained in DMEM (Gibco, USA).

Techniques: Incubation, Lucia Comet Assay, Imaging, Control

Cell viability of HepG2 cells after treatment with graphene oxide, antisense miRNA-21 and GO–antisense miRNA-21 nanosystems. ( A ) Viability (%) after 24 h exposure to increasing concentrations of GO (5–50 µg/mL), measured by MTT assay, untreated cells were used as control. ( B ) Analysis of cell viability after 24 h following treatment with GO (10 µg/mL), antisense miRNA-21 (5 pmol/mL), and GO–antisense miRNA-21 (10 µg/mL GO + 5 pmol/mL antisense). Data are expressed as percentage relative to untreated control cells. Data are presented as mean ± SD (n = 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test. Asterisks indicate statistical significance: **** p < 0.0001. Horizontal lines above bars indicate the groups being compared. Abbreviation: Electroporation CTR: electroporation control.

Journal: International Journal of Molecular Sciences

Article Title: Graphene-Based Nanosystem for Targeted Delivery of Anti-Sense miRNA-21 on Hepatocellular Carcinoma Cells

doi: 10.3390/ijms27020975

Figure Lengend Snippet: Cell viability of HepG2 cells after treatment with graphene oxide, antisense miRNA-21 and GO–antisense miRNA-21 nanosystems. ( A ) Viability (%) after 24 h exposure to increasing concentrations of GO (5–50 µg/mL), measured by MTT assay, untreated cells were used as control. ( B ) Analysis of cell viability after 24 h following treatment with GO (10 µg/mL), antisense miRNA-21 (5 pmol/mL), and GO–antisense miRNA-21 (10 µg/mL GO + 5 pmol/mL antisense). Data are expressed as percentage relative to untreated control cells. Data are presented as mean ± SD (n = 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test. Asterisks indicate statistical significance: **** p < 0.0001. Horizontal lines above bars indicate the groups being compared. Abbreviation: Electroporation CTR: electroporation control.

Article Snippet: HepG2 human liver carcinoma cells (ATCC ® HB-8065TM) were cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin.

Techniques: MTT Assay, Control, Electroporation

Evaluation of cellular uptake efficiency of GO, antisense miRNA-21, and GO–antisense miRNA-21 nanosystems in HepG2 cells. ( A ) Flow cytometry analysis of HepG2 cells treated with FITC-labeled GO, antisense miRNA-21, and GO–antisense miRNA-21 for 24 h. The x-axis represents FITC fluorescence intensity, corresponding to the intracellular uptake level of the labeled constructs. Fluorescence intensity was measured to determine the proportion of FITC-positive cells. ( B ) Confocal microscopy imaging of FITC-labeled (green color) constructs in HepG2 cells after 24 h incubation. Cells were counterstained with DAPI (nuclei- blue color) and ActinRed (cytoskeleton-red color). Scale bar: 50 µm. ( C ) Quantitative analysis by flow cytometry showing the percentage of FITC-positive cells after treatment with GO, antisense miRNA-21, and the GO-antisense complex. Data are presented as mean ± SEM.

Journal: International Journal of Molecular Sciences

Article Title: Graphene-Based Nanosystem for Targeted Delivery of Anti-Sense miRNA-21 on Hepatocellular Carcinoma Cells

doi: 10.3390/ijms27020975

Figure Lengend Snippet: Evaluation of cellular uptake efficiency of GO, antisense miRNA-21, and GO–antisense miRNA-21 nanosystems in HepG2 cells. ( A ) Flow cytometry analysis of HepG2 cells treated with FITC-labeled GO, antisense miRNA-21, and GO–antisense miRNA-21 for 24 h. The x-axis represents FITC fluorescence intensity, corresponding to the intracellular uptake level of the labeled constructs. Fluorescence intensity was measured to determine the proportion of FITC-positive cells. ( B ) Confocal microscopy imaging of FITC-labeled (green color) constructs in HepG2 cells after 24 h incubation. Cells were counterstained with DAPI (nuclei- blue color) and ActinRed (cytoskeleton-red color). Scale bar: 50 µm. ( C ) Quantitative analysis by flow cytometry showing the percentage of FITC-positive cells after treatment with GO, antisense miRNA-21, and the GO-antisense complex. Data are presented as mean ± SEM.

Article Snippet: HepG2 human liver carcinoma cells (ATCC ® HB-8065TM) were cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin.

Techniques: Flow Cytometry, Labeling, Fluorescence, Construct, Confocal Microscopy, Imaging, Incubation

Quantitative real-time PCR analysis of miRNA expression and pro-inflammatory and tumor-related gene expression in HepG2 cells. ( A ) Transcriptional levels of miRNA-21 in untreated HepG2 cells and in cells treated with GO (10 µg/mL) and with Antisense miRNA-21 (5 pmol/mL) for 24 h. ( B ) Transcriptional levels of IL-8, MCP-1, ICAM-1, TIMP-2 and NF-κB in HepG2 cells. The cells were untreated and treated with GO (10 µg/mL) and GO-antisense miRNA-21 (GO 10 µg/mL + antisense miRNA-21 5 pmol/mL) for 24 h. After, the cells were collected for total RNA extraction. The transcriptional levels of the considered genes were analyzed by calculating the value of 2 −ΔΔCt . The assay was performed in triplicate ± SD and expressed as a fold change over the housekeeping genes. **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Graphene-Based Nanosystem for Targeted Delivery of Anti-Sense miRNA-21 on Hepatocellular Carcinoma Cells

doi: 10.3390/ijms27020975

Figure Lengend Snippet: Quantitative real-time PCR analysis of miRNA expression and pro-inflammatory and tumor-related gene expression in HepG2 cells. ( A ) Transcriptional levels of miRNA-21 in untreated HepG2 cells and in cells treated with GO (10 µg/mL) and with Antisense miRNA-21 (5 pmol/mL) for 24 h. ( B ) Transcriptional levels of IL-8, MCP-1, ICAM-1, TIMP-2 and NF-κB in HepG2 cells. The cells were untreated and treated with GO (10 µg/mL) and GO-antisense miRNA-21 (GO 10 µg/mL + antisense miRNA-21 5 pmol/mL) for 24 h. After, the cells were collected for total RNA extraction. The transcriptional levels of the considered genes were analyzed by calculating the value of 2 −ΔΔCt . The assay was performed in triplicate ± SD and expressed as a fold change over the housekeeping genes. **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05.

Article Snippet: HepG2 human liver carcinoma cells (ATCC ® HB-8065TM) were cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Gene Expression, RNA Extraction

Pb Lig1 is essential for parasite development in the liver. ( A ) HepG2 cells were infected with either UIS4/Flp or Pb Lig1 cKO sporozoites. Infected cultures were fixed at the indicated time points post-infection and immunostained with an anti-UIS4 antibody to visualize EEFs. ( B ) EEF area was comparable at 24 hpi ( P = 0.2500) and 36 hpi ( P = 0.1840), but was significantly reduced in Pb Lig1 cKO parasites at 55 hpi (**** P < 0.0001). Data represent the mean ± SEM from three independent experiments. ( C ) Quantification of EEF numbers at 24, 36, and 55 hpi revealed no significant differences between UIS4/Flp and Pb Lig1 cKO parasites (24 hpi, P = 0.6063; 36 hpi, P = 0.9036; 55 hpi, P = 0.9929; one-way ANOVA).

Journal: mSphere

Article Title: Plasmodium DNA ligase I is essential for parasite blood- and liver-stage development

doi: 10.1128/msphere.00674-25

Figure Lengend Snippet: Pb Lig1 is essential for parasite development in the liver. ( A ) HepG2 cells were infected with either UIS4/Flp or Pb Lig1 cKO sporozoites. Infected cultures were fixed at the indicated time points post-infection and immunostained with an anti-UIS4 antibody to visualize EEFs. ( B ) EEF area was comparable at 24 hpi ( P = 0.2500) and 36 hpi ( P = 0.1840), but was significantly reduced in Pb Lig1 cKO parasites at 55 hpi (**** P < 0.0001). Data represent the mean ± SEM from three independent experiments. ( C ) Quantification of EEF numbers at 24, 36, and 55 hpi revealed no significant differences between UIS4/Flp and Pb Lig1 cKO parasites (24 hpi, P = 0.6063; 36 hpi, P = 0.9036; 55 hpi, P = 0.9929; one-way ANOVA).

Article Snippet: Human liver hepatocellular carcinoma (HepG2) cells (ATCC) were used for in vitro infection of sporozoites.

Techniques: Infection

Pb Lig1 is essential for nuclear division and merozoite development. ( A ) HepG2 cells infected with UIS4/Flp or Pb Lig1 cKO sporozoites were fixed at 62 hpi and immunostained with anti-UIS4 and anti-MSP1 antibodies. UIS4/Flp parasites exhibited robust MSP1 staining, indicative of merozoite formation, whereas Pb Lig1 cKO parasites lacked MSP1 signal, suggesting a block in merozoite development. ( B ) Quantification of nuclear number at 55 hpi. Nuclei were manually counted in ImageJ-analyzed images. Pb Lig1 cKO EEFs displayed a significantly reduced number of nuclei compared to UIS4/Flp (**** P < 0.0001, unpaired t -test). ( C ) Quantification of DNA content using Hoechst 33342 staining at 36 hpi and 55 hpi. UIS4/Flp and Pb Lig1 cKO EEFs were fixed and immunostained with anti-UIS4 and anti-MSP1 antibodies. Nuclear DNA within the PV was quantified by calculating corrected total cell fluorescence (CTCF) from Hoechst signal. Data represent 43 (UIS4/Flp 36 hpi), 47 ( Pb Lig1 cKO 36 hpi), 60 (UIS4/Flp 55 hpi), and 60 ( Pb Lig1 cKO 55 hpi) individual EEFs. No significant difference in CTCF was observed at 36 hpi ( P = 0.0693), while a significant reduction was detected in Pb Lig1 cKO parasites at 55 hpi (**** P < 0.0001, unpaired t -test), n.s., not significant. Data are shown as mean ± SD from two independent biological replicates.

Journal: mSphere

Article Title: Plasmodium DNA ligase I is essential for parasite blood- and liver-stage development

doi: 10.1128/msphere.00674-25

Figure Lengend Snippet: Pb Lig1 is essential for nuclear division and merozoite development. ( A ) HepG2 cells infected with UIS4/Flp or Pb Lig1 cKO sporozoites were fixed at 62 hpi and immunostained with anti-UIS4 and anti-MSP1 antibodies. UIS4/Flp parasites exhibited robust MSP1 staining, indicative of merozoite formation, whereas Pb Lig1 cKO parasites lacked MSP1 signal, suggesting a block in merozoite development. ( B ) Quantification of nuclear number at 55 hpi. Nuclei were manually counted in ImageJ-analyzed images. Pb Lig1 cKO EEFs displayed a significantly reduced number of nuclei compared to UIS4/Flp (**** P < 0.0001, unpaired t -test). ( C ) Quantification of DNA content using Hoechst 33342 staining at 36 hpi and 55 hpi. UIS4/Flp and Pb Lig1 cKO EEFs were fixed and immunostained with anti-UIS4 and anti-MSP1 antibodies. Nuclear DNA within the PV was quantified by calculating corrected total cell fluorescence (CTCF) from Hoechst signal. Data represent 43 (UIS4/Flp 36 hpi), 47 ( Pb Lig1 cKO 36 hpi), 60 (UIS4/Flp 55 hpi), and 60 ( Pb Lig1 cKO 55 hpi) individual EEFs. No significant difference in CTCF was observed at 36 hpi ( P = 0.0693), while a significant reduction was detected in Pb Lig1 cKO parasites at 55 hpi (**** P < 0.0001, unpaired t -test), n.s., not significant. Data are shown as mean ± SD from two independent biological replicates.

Article Snippet: Human liver hepatocellular carcinoma (HepG2) cells (ATCC) were used for in vitro infection of sporozoites.

Techniques: Infection, Staining, Blocking Assay, Fluorescence

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human liver carcinoma cell line hepg2 (ATCC)

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1) Product Images from "Establishment and Validation of a Cell-Based Relative Potency Method for Respiratory Syncytial Virus mRNA Vaccine Drug Substance"

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